BCL6 Recombinant Mouse Monoclonal Antibody [A6C8-R]
cat.: HA601084
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A6C8-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.014ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 79 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human BCL-6 aa 301-500 / 706. |
| Positive control: | Human B lymphocytoma tissue, human lymph nodes tissue, Raji. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IHC-P IF-Cell |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P41182 Human |
| Alternative names: | B cell CLL/lymphoma 6 B cell lymphoma 6 protein B-cell lymphoma 5 protein B-cell lymphoma 6 protein BCL 5 Bcl 6 BCL-5 BCL-6 BCL5 BCL6 BCL6_HUMAN BCL6A cys his2 zinc finger transcription factor cys-his2 zinc finger transcription factor LAZ 3 LAZ 3 protein LAZ3 Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3) Lymphoma associated zinc finger gene on chromosome 3 Protein LAZ-3 ZBTB 27 ZBTB27 Zinc finger and BTB domain containing protein 27 Zinc finger and BTB domain-containing protein 27 (ZBTB27) Zinc finger and BTB domain-containing protein 27 Zinc Finger Protein 51 (ZNF51) Zinc finger protein 51 zinc finger transcription factor BCL6S ZNF 51 ZNF51 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human B lymphocytoma tissue with Mouse anti-BCL6 antibody (HA601084) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601084) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Mouse anti-BCL6 antibody (HA601084) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601084) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunocytochemistry analysis of Raji cells labeling BCL6 with Mouse anti-BCL6 antibody (HA601084) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-BCL6 antibody (HA601084) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.