TMED9 Mouse Monoclonal Antibody [A9A7]
cat.: HA601086
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A9A7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human TMED9 aa 31-220 / 235. |
| Positive control: | Hela cell lysate, THP-1 cell lysate, A549 cell lysate, MCF-7 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, mouse lung tissue lysate, HepG2 cell lysate, LO2 cell lysate, human liver tissue lysate, rat liver tissue lysate, mouse liver tissue. |
| Subcellular location: | Endoplasmic reticulum membrane, Golgi apparatus, Endoplasmic reticulum-Golgi intermediate compartment membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000-1:10,000 1:1,000 |
| Uniprot #: | SwissProt: Q9BVK6 Human | Q99KF1 Mouse | Q5I0E7 Rat |
| Alternative names: | Transmembrane emp24 domain-containing protein 9 GMP25 Glycoprotein 25L2 p24 family protein alpha-2 (p24alpha2) p25 TMED9 GP25L2 |
Images
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Fig1:
Western blot analysis of TMED9 on different lysates with Mouse anti-TMED9 antibody (HA601086) at 1/5,000 dilution. Lane 1: Hela cell lysate (10 µg/Lane) Lane 2: THP-1 cell lysate (10 µg/Lane) Lane 3: A549 cell lysate (10 µg/Lane) Lane 4: MCF-7 cell lysate (10 µg/Lane) Lane 5: PC-12 cell lysate (10 µg/Lane) Lane 6: NIH/3T3 cell lysate (10 µg/Lane) Lane 7: Mouse liver tissue lysate (20 µg/Lane) Lane 8: Mouse lung tissue lysate (20 µg/Lane) Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601086) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of TMED9 on different lysates with Mouse anti-TMED9 antibody (HA601086) at 1/10,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: LO2 cell lysate (10 µg/Lane) Lane 3: Human liver tissue lysate (20 µg/Lane) Lane 4: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601086) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-TMED9 antibody (HA601086) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601086) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.