CD204 Mouse Monoclonal Antibody [A8G2]
cat.: HA601090
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A8G2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human CD204 aa 51-250 / 451. |
| Positive control: | THP-1 cell lysate treated with 80nM PMA for 72 hours, human liver tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:5,000-1:10,000 1:500 |
| Uniprot #: | SwissProt: P21757 Human |
| Alternative names: | CD204 CD204 antigen CD24 Macrophage acetylated LDL receptor I and II Macrophage scavenger receptor 1 Macrophage scavenger receptor type III Macrophage scavenger receptor types I and II Msr 1 MSR1 MSRE_HUMAN phSR1 phSR2 SCARA 1 SCARA1 Scavenger receptor class A member 1 Scavenger receptor class A, member 1 Scavenger receptor type A Scvr SR A SRA |
Images
|
Fig1:
Western blot analysis of CD204 on different lysates with Mouse anti-CD204 antibody (HA601090) at 1/5,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 cell lysate treated with 80nM PMA for 72 hours Lysates/proteins at 10 µg/Lane. Predicted band size: 50 kDa Observed band size: 75 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601090) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD204 antibody (HA601090) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601090) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.