c-Met Mouse Monoclonal Antibody [A9A5]
cat.: HA601093
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A9A5 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 156 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human c-Met aa 1,341-1,390 / 1,390. |
| Positive control: | A549 cell lysate, HepG2 cell lysate, human lung carcinoma tissue. |
| Subcellular location: | Membrane; Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:400 |
| Uniprot #: | SwissProt: P08581 Human |
| Alternative names: | Hepatocyte growth factor receptor EC:2.7.10.1 HGF receptor HGF/SF receptor Proto-oncogene c-Met Scatter factor receptor (SF receptor) Tyrosine-protein kinase Met |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Mouse anti-c-Met antibody (HA601093) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601093) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Western blot analysis of c-Met on different lysates with Mouse anti-c-Met antibody (HA601093) at 1/1,000 dilution. Lane 1: HEK293T (Human embryonic kidney cell) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601093, 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 155.5 kDa Observed band size: 155 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.