Kappa Light Chain Mouse Monoclonal Antibody [A8H10]
cat.: HA601099
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Tissue, WB |
| Clonality: | Monoclonal |
| Clone number: | A8H10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 12 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant full protein within human IGKC. |
| Positive control: | Human plasma lysates, human tonsil tissue, human appendix tissue. |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
IHC-P IF-Tissue WB |
1:500 1:50 1:1,000 |
| Uniprot #: | SwissProt: P01834 Human |
| Alternative names: | HCAK 1 HCAK1 Ig kappa chain C region IGKC IGKCD Immunoglobulin InV Immunoglobulin kappa constant Immunoglobulin kappa constant region Immunoglobulin kappa light chain Immunoglobulin kappa light chain constant region Immunoglobulin KM Kappa 1 immunoglobulin light chain kappa light chain Km MGC111575 MGC62011 MGC72072 MGC88770 MGC88771 MGC88809 |
Images
|
Fig1:
Western blot analysis of Kappa Light Chain on human plasma lysates with Mouse anti-Kappa Light Chain antibody (HA601099) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 12 kDa Observed band size: 25 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601099) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Kappa Light Chain antibody (HA601099) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601099) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-Kappa Light Chain antibody (HA601099) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601099) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Kappa Light Chain with Mouse anti-Kappa Light Chain antibody (HA601099) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601099, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.