ASS1 Recombinant Mouse Monoclonal Antibody [11F1-RA]
cat.: HA601101
| Product Type: | Recombinant Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 11F1-RA |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within Human ASS1 aa 1-412 / 412. |
| Positive control: | HepG2 cell lysate, THP-1 cell lysate, SiHa cell lysate, rat kidney tissue lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat kidney tissue, human kidney tissue, MCF-7. |
| Subcellular location: | Cytoplasm |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:5,000-1:20,000 1:1,000 1:200 |
| Uniprot #: | SwissProt: P00966 Human | P16460 Mouse | P09034 Rat |
| Alternative names: | Argininosuccinate synthase 1 Argininosuccinate synthase Argininosuccinate synthetase 1 ASS Ass-1 ass1 ASSA ASSY_HUMAN Citrulline aspartate ligase Citrulline--aspartate ligase CTLN1 |
Images
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Fig1:
Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (HA601101) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (10 µg/Lane) Lane 2: THP-1 cell lysate (10 µg/Lane) Lane 3: SiHa cell lysate (10 µg/Lane) Lane 4: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601101) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (HA601101) at 1/20,000 dilution. Lane 1: Mouse liver tissue lysate (20 µg/Lane) Lane 2: Mouse kidney tissue lysate (20 µg/Lane) Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601101) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (HA601101) at 1/2,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD ASS1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 45 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601101) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-ASS1 antibody (HA601101) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601101) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-ASS1 antibody (HA601101) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601101) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunocytochemistry analysis of MCF-7 cells labeling ASS1 with Mouse anti-ASS1 antibody (HA601101) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-ASS1 antibody (HA601101) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.