Chromogranin A Recombinant Mouse Monoclonal Antibody [PD01-39]
cat.: HA601103
Product Type: Recombinant Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: IHC-P
Clonality: Monoclonal
Clone number: PD01-39
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG1
Immunogen: Recombinant full length protein corresponding to Human Chromogranin A.
Positive control: Human pancreas tissue, human thyroid tissue, human atypical carcinoid tissue, human medullary thyroid carcinoma tissue, human appendix tissue.
Subcellular location: Cytoplasmic vesicle, Secreted.
Recommended Dilutions:
  IHC-P
1:1,000-1:15,000
Uniprot #: SwissProt: P10645 Human
Alternative names: beta Granin betagranin (N-terminal fragment of chromogranin A) catestatin CgA CHG A Chga chromofungin Chromogranin A parathyroid secretory protein 1 Chromogranin A precursor ChromograninA CMGA_HUMAN ER-37 Pancreastatin Parastatin Parathyroid secretory protein 1 Pituitary secretory protein I Secretory protein I SP I SP-I SP1 SPI Vasostatin Vasostatin I Vasostatin II
Images
HA601103_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-Chromogranin A antibody (HA601103) at 1/15,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601103) at 1/15,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601103_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Mouse anti-Chromogranin A antibody (HA601103) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601103) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601103_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human atypical carcinoid tissue with Mouse anti-Chromogranin A antibody (HA601103) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601103) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601103_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human medullary thyroid carcinoma tissue with Mouse anti-Chromogranin A antibody (HA601103) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601103) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601103_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-Chromogranin A antibody (HA601103) at 1/10,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601103) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601103_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human mesothelioma tissue (Negative control) with Mouse anti-Chromogranin A antibody (HA601103) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601103) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601103_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma tissue (Negative control) with Mouse anti-Chromogranin A antibody (HA601103) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601103) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.