HIV1 p24 Recombinant Mouse Monoclonal Antibody [12G2-R]
cat.: HA601105
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | HIV1 |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | 12G2-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within HIV1 p24 protein aa 133-363 (Catalog#HA210278). |
| Positive control: | Recombanint HIV1 p24 protein. |
| Subcellular location: | Virion. |
| Recommended Dilutions:
ELISA(Det) |
5-20 ng/ml |
| Uniprot #: | SwissProt: P12497 HIV1 |
| Alternative names: | CA Capsid protein p24 HIV-1 Gag p24 HIV1gp1 Human immunodeficiency virus 1 Human immunodeficiency virus type 1 p24 |
Images
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Fig1:
Standard curve of p24 matched pair antibodies: Sandwich ELISA analysis of p24 matched piar antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA601104 [12G4-R] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150ul 1% BSA/PBST blocking buffer, and incubated with serial diluted recombinant p24 protein starting from 1000pg/ml to 15.6pg/ml for 1 hours at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody HA601105 [12G2-R] (Biotin, 1:2,000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. Capture/detect antibodies, recombinant p24 protein, Streptavidin-HRP are diluted in 1% BSA/PBST. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.