FIP200 Mouse Monoclonal Antibody [A9A9]
cat.: HA601106
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | A9A9 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.21ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 183 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human RB1CC1 aa 651-800 / 1,594. |
| Positive control: | Hela cell lysate, MCF-7 cell lysate, 293T cell lysate, rat kidney tissue, human kidney tissue, Hela, MCF7, NIH/3T3. |
| Subcellular location: | Nucleus, Cytoplasm, Cytoplasm, cytosol, Lysosome. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:1,000 1:200-1:1,000 1:200 1:1,000 |
| Uniprot #: | SwissProt: Q8TDY2 Human | Q9ESK9 Mouse Entrez Gene: 312927 Rat |
| Alternative names: | FAK family kinase-interacting protein of 200 kDa FIP200 RB1-inducible coiled-coil protein 1 RB1CC1 RBCC1_HUMAN |
Images
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Fig1:
Western blot analysis of FIP200 on different lysates with Mouse anti-FIP200 antibody (HA601106) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 µg/Lane) Lane 2: MCF-7 cell lysate (30 µg/Lane) Lane 3: 293T cell lysate (30 µg/Lane) Predicted band size: 183 kDa Observed band size: 250/150 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601106) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-FIP200 antibody (HA601106) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601106) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-FIP200 antibody (HA601106) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601106) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of Hela cells labeling FIP200 with Mouse anti-FIP200 antibody (HA601106) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-FIP200 antibody (HA601106) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Flow cytometric analysis of MCF7 cells labeling FIP200. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601106, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
Flow cytometric analysis of NIH/3T3 cells labeling FIP200. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601106, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.