FIP200 Mouse Monoclonal Antibody [A9A10]
cat.: HA601107
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A9A10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1.17ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 183 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human RB1CC1 aa 651-800 / 1,594. |
| Positive control: | Hela cell lysate, MG-63 cell lysate, MCF-7 cell lysate, 293T cell lysate, K562 cell lysate, A431 cell lysate, Jurkat cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate. |
| Subcellular location: | Nucleus, Cytoplasm, Cytoplasm, cytosol, Lysosome. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q8TDY2 Human | Q9ESK9 Mouse |
| Alternative names: | FAK family kinase-interacting protein of 200 kDa FIP200 RB1-inducible coiled-coil protein 1 RB1CC1 RBCC1_HUMAN |
Images
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Fig1:
Western blot analysis of FIP200 on different lysates with Mouse anti-FIP200 antibody (HA601107) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: MG-63 cell lysate Lane 3: MCF-7 cell lysate Lane 4: 293T cell lysate Lane 5: K562 cell lysate Lane 6: A431 cell lysate Lane 7: Jurkat cell lysate Lane 8: HepG2 cell lysate Lane 9: NIH/3T3 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 183 kDa Observed band size: 250/150 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601107) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.