ACSL1 Mouse Monoclonal Antibody [A9B5]
cat.: HA601112
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: A9B5
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 78 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human aa 151-350 / 698.
Positive control: Rat kidney tissue, human liver tissue, mouse heart tissue, Raji cell lysate, HepG2 cell lysate, LO2 cell lysate, A431 cell lysate.
Subcellular location: Mitochondrion outer membrane, Peroxisome membrane, Microsome membrane, Endoplasmic reticulum membrane.
Recommended Dilutions:
  WB
  IHC-P

1:1,000-1:5,000
1:200-1:1,000
Uniprot #: SwissProt: P33121 Human | P41216 Mouse | P18163 Rat
Alternative names: ACS 1 ACS1 ACSL 1 ACSL1 ACSL1_HUMAN Acyl CoA synthetase 1 Acyl CoA synthetase long chain family member 1 Acyl-CoA synthetase 1 FACL 1 FACL 2 FACL1 FACL2 Fatty acid Coenzyme A ligase long chain 1 Fatty acid Coenzyme A ligase long chain 2 LACS 1 LACS 2 LACS LACS1 LACS2 Lignoceroyl CoA synthase Long chain acyl CoA synthetase 1 Long chain acyl CoA synthetase 2 Long chain fatty acid CoA ligase 1 Long chain fatty acid CoA ligase 2 Long chain fatty acid coenzyme A ligase 1 Long-chain acyl-CoA synthetase 1 Long-chain acyl-CoA synthetase 2 Long-chain fatty acid-CoA ligase 2 Long-chain-fatty-acid--CoA ligase 1 Palmitoyl CoA ligase 1 Palmitoyl CoA ligase 2 Palmitoyl-CoA ligase 1 Palmitoyl-CoA ligase 2 Paltimoyl CoA ligase 1
Images
HA601112_1.jpg Fig1: Western blot analysis of ACSL1 on different lysates with Mouse anti-ACSL1 antibody (HA601112) at 1/5,000 dilution.

Lane 1: A549-si NT cell lysate
Lane 2: A549-si ACSL1 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 78 kDa
Observed band size: 70 kDa

Exposure time: 2 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601112) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
HA601112_2.jpg Fig2: Western blot analysis of ACSL1 on different lysates with Mouse anti-ACSL1 antibody (HA601112) at 1/1,000 dilution.

Lane 1: Raji cell lysate
Lane 2: HepG2 cell lysate
Lane 3: LO2 cell lysate
Lane 4: A431 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 78 kDa
Observed band size: 78 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601112) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.
HA601112_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-ACSL1 antibody (HA601112) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601112) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601112_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ACSL1 antibody (HA601112) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601112) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601112_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Mouse anti-ACSL1 antibody (HA601112) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601112) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.