Product Type: | Mouse monoclonal IgG1, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P |
Clonality: | Monoclonal |
Clone number: | A9B5 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 78 kDa |
Isotype: | IgG1 |
Immunogen: | Recombinant protein within human aa 151-350 / 698. |
Positive control: | Rat kidney tissue, human liver tissue, mouse heart tissue, Raji cell lysate, HepG2 cell lysate, LO2 cell lysate, A431 cell lysate. |
Subcellular location: | Mitochondrion outer membrane, Peroxisome membrane, Microsome membrane, Endoplasmic reticulum membrane. |
Recommended Dilutions:
WB IHC-P |
1:1,000-1:5,000 1:200-1:1,000 |
Uniprot #: | SwissProt: P33121 Human | P41216 Mouse | P18163 Rat |
Alternative names: | ACS 1 ACS1 ACSL 1 ACSL1 ACSL1_HUMAN Acyl CoA synthetase 1 Acyl CoA synthetase long chain family member 1 Acyl-CoA synthetase 1 FACL 1 FACL 2 FACL1 FACL2 Fatty acid Coenzyme A ligase long chain 1 Fatty acid Coenzyme A ligase long chain 2 LACS 1 LACS 2 LACS LACS1 LACS2 Lignoceroyl CoA synthase Long chain acyl CoA synthetase 1 Long chain acyl CoA synthetase 2 Long chain fatty acid CoA ligase 1 Long chain fatty acid CoA ligase 2 Long chain fatty acid coenzyme A ligase 1 Long-chain acyl-CoA synthetase 1 Long-chain acyl-CoA synthetase 2 Long-chain fatty acid-CoA ligase 2 Long-chain-fatty-acid--CoA ligase 1 Palmitoyl CoA ligase 1 Palmitoyl CoA ligase 2 Palmitoyl-CoA ligase 1 Palmitoyl-CoA ligase 2 Paltimoyl CoA ligase 1 |
Fig1:
Western blot analysis of ACSL1 on different lysates with Mouse anti-ACSL1 antibody (HA601112) at 1/5,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si ACSL1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 78 kDa Observed band size: 70 kDa Exposure time: 2 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601112) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of ACSL1 on different lysates with Mouse anti-ACSL1 antibody (HA601112) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: HepG2 cell lysate Lane 3: LO2 cell lysate Lane 4: A431 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 78 kDa Observed band size: 78 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601112) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |
Fig3:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-ACSL1 antibody (HA601112) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601112) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ACSL1 antibody (HA601112) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601112) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Mouse anti-ACSL1 antibody (HA601112) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601112) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |