POMC Recombinant Mouse Monoclonal Antibody [PD01-40]
cat.: HA601113
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P, IF-Tissue, IHC-Fr, IF-Cell, WB |
| Clonality: | Monoclonal |
| Clone number: | PD01-40 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human POMC aa 1-150 / 267. |
| Positive control: | Mouse pituitary tissue, rat pituitary tissue, mouse hypothalamus (arcuate nucleus) tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P IF-Tissue IHC-Fr IF-Cell |
1:5,000 1:10,000-1:20,000 1:5,000 1:1,000 1:500 |
| Uniprot #: | SwissProt: P01189 Human | P01193 Mouse | P01194 Rat |
| Alternative names: | ACTH Adrenocorticotropic hormone Adrenocorticotropin alpha melanocyte stimulating hormone Alpha MSH Alpha-MSH beta endorphin Beta LPH beta melanocyte stimulating hormone Beta MSH Beta-LPH Beta-MSH CLIP COLI_HUMAN Corticotropin like intermediary peptide Corticotropin lipotropin Corticotropin-lipotropin Gamma MSH Gamma-LPH Gamma-MSH Lipotropin beta Lipotropin gamma LPH Melanotropin alpha Melanotropin Melanotropin beta Melanotropin gamma met enkephalin Met-enkephalin MSH NPP POC POMC pro ACTH endorphin Proopiomelanocortin Proopiomelanocortin preproprotein |
Images
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Fig1:
Application: Immunofluorescence (IHC-Fr) Species: Rat Tissue: Pituitary Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA601113, 1/1,000, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. |
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Fig2:
Application: Immunofluorescence (IHC-Fr) Species: Rat Tissue: Pituitary Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: HA601113, 1/1,000, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Mouse anti-POMC antibody (HA601113) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601113) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Mouse anti-POMC antibody (HA601113) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601113) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunofluorescence analysis of paraffin-embedded mouse pituitary tissue labeling POMC with Mouse anti-POMC antibody (HA601113) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601113, green) at 1/5,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig6:
Immunofluorescence analysis of paraffin-embedded mouse hypothalamus (arcuate nucleus) tissue labeling POMC with Mouse anti-POMC antibody (HA601113) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601113, green) at 1/5,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig7:
Application: Immunocytochemistry (IF-cell) Species: Mouse Sample: AtT-20 (Mouse pituitary tumor cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA601113, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig8:
Western blot analysis of POMC on different lysates with Mouse anti-POMC antibody (HA601113) at 1/5,000 dilution. Lane 1: Mouse pituitary tissue lysate Lane 2: Mouse liver tissue lysate (negative) Lane 3: Rat pituitary tissue lysate Lane 4: Rat liver tissue lysate (negative) Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; ECL: K1801 POMC is not expressed in normal liver tissue (PMID: 6177687). Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601113, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 29 kDa Observed band size: 16-35 kDa |
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Fig9:
Immunohistochemical analysis of paraffin-embedded human pituitary tissue with Mouse anti-POMC antibody (HA601113) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601113) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.