POMC Recombinant Mouse Monoclonal Antibody [PD01-40]
cat.: HA601113
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PD01-40 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human POMC aa 1-150 / 267. |
| Positive control: | Mouse pituitary tissue, rat pituitary tissue, mouse hypothalamus (arcuate nucleus) tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P IF-Tissue |
1:4,000 1:5,000 |
| Uniprot #: | SwissProt: P01189 Human | P01193 Mouse | P01194 Rat |
| Alternative names: | ACTH Adrenocorticotropic hormone Adrenocorticotropin alpha melanocyte stimulating hormone Alpha MSH Alpha-MSH beta endorphin Beta LPH beta melanocyte stimulating hormone Beta MSH Beta-LPH Beta-MSH CLIP COLI_HUMAN Corticotropin like intermediary peptide Corticotropin lipotropin Corticotropin-lipotropin Gamma MSH Gamma-LPH Gamma-MSH Lipotropin beta Lipotropin gamma LPH Melanotropin alpha Melanotropin Melanotropin beta Melanotropin gamma met enkephalin Met-enkephalin MSH NPP POC POMC pro ACTH endorphin Proopiomelanocortin Proopiomelanocortin preproprotein |
Images
|
Fig1:
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Mouse anti-POMC antibody (HA601113) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601113) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Mouse anti-POMC antibody (HA601113) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601113) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunofluorescence analysis of paraffin-embedded mouse pituitary tissue labeling POMC with Mouse anti-POMC antibody (HA601113) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601113, green) at 1/5,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
|
Fig4:
Immunofluorescence analysis of paraffin-embedded mouse hypothalamus (arcuate nucleus) tissue labeling POMC with Mouse anti-POMC antibody (HA601113) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601113, green) at 1/5,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.