POMC Recombinant Mouse Monoclonal Antibody [PD01-40]
cat.: HA601113
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PD01-40 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human POMC aa 1-150 / 267. |
| Positive control: | Rat pituitary tissue, mouse pituitary tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P |
1:4,000 |
| Uniprot #: | SwissProt: P01189 Human | P01193 Mouse | P01194 Rat |
| Alternative names: | ACTH Adrenocorticotropic hormone Adrenocorticotropin alpha melanocyte stimulating hormone Alpha MSH Alpha-MSH beta endorphin Beta LPH beta melanocyte stimulating hormone Beta MSH Beta-LPH Beta-MSH CLIP COLI_HUMAN Corticotropin like intermediary peptide Corticotropin lipotropin Corticotropin-lipotropin Gamma MSH Gamma-LPH Gamma-MSH Lipotropin beta Lipotropin gamma LPH Melanotropin alpha Melanotropin Melanotropin beta Melanotropin gamma met enkephalin Met-enkephalin MSH NPP POC POMC pro ACTH endorphin Proopiomelanocortin Proopiomelanocortin preproprotein |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Mouse anti-POMC antibody (HA601113) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601113) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Mouse anti-POMC antibody (HA601113) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601113) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.