CD74 Recombinant Mouse Monoclonal Antibody [PDM0-22]
cat.: HA601117
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PDM0-22 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human CD74 73-296 (Extracellular). |
| Positive control: | Daudi cell lysate, Raji cell lysate, LOVO, human B-cell lymphoma tissue, human tonsil tissue, human spleen tissue, human prostate cancer tissue. |
| Subcellular location: | Endoplasmic reticulum membrane, trans-Golgi network, cell membrane, lysosome, endosome. |
| Recommended Dilutions:
WB IHC-P IF-Cell IF-Tissue |
1:1,000 1:1,000 1:50 1:1,000 |
| Uniprot #: | SwissProt: P04233 Human |
| Alternative names: | CD 74 CD74 CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) CD74 antigen CD74 molecule CD74 molecule, major histocompatibility complex, class II invariant chain CLIP DHLAG Gamma chain of class II antigens HG2A_HUMAN HLA class II histocompatibility antigen gamma chain HLA DR antigens associated invariant chain HLA DR gamma HLA-DR antigens-associated invariant chain HLA-DR-gamma HLADG HLADR antigens associated invariant chain Ia antigen associated invariant chain Ia antigen-associated invariant chain Ia associated invariant chain Ia gamma Ii Invariant polypeptide of major histocompatibility complex class II antigen associated la-gamma Major histocompatibility complex class II invariant chain MHC HLA DR gamma chain MHC HLA-DR gamma chain p33 p35 Protein 41 |
Images
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Fig1:
Western blot analysis of CD74 on different lysates with Mouse anti-CD74 antibody (HA601117) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601117) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of LOVO cells labeling CD74 with Mouse anti-CD74 antibody (HA601117) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-CD74 antibody (HA601117) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Mouse anti-CD74 antibody (HA601117) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601117) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD74 antibody (HA601117) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601117) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD74 antibody (HA601117) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601117) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Mouse anti-CD74 antibody (HA601117) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601117) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Mouse anti-CD74 antibody (HA601117) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601117) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Application: IF-Tissue Species: Human Site: B-cell lymphoma Sample: Paraffin-embedded section Antibody concentration: 1/1,000 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.