CD79a Recombinant Mouse Monoclonal Antibody [PDM0-23]
cat.: HA601118
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PDM0-23 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human CD79a aa 33-143 (Extracellular). |
| Positive control: | Daudi cell lysates, Ramos cell lysates, Raji cell lysates, Jurkat cell lysates, Human tonsil tissue, human appendix tissue, human lymph nodes tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P11912 Human |
| Alternative names: | B lymphocyte-specific MB1 protein B-cell antigen receptor complex-associated protein alpha chain CD 79a CD79a CD79a antigen (immunoglobulin-associated alpha) CD79A antigen CD79a molecule, immunoglobulin-associated alpha CD79A_HUMAN Ig alpha Ig-alpha IGA IgM-alpha Immunoglobulin-associated alpha Ly54 MB-1 membrane glycoprotein MB1 Membrane-bound immunoglobulin-associated protein Surface IgM-associated protein |
Images
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Fig1:
Western blot analysis of CD79a on different lysates with Rabbit anti-CD79a antibody (HA601118) at 1/2,000 dilution. Lane 1: Daudi (Human Burkitt's lymphoma cell) cell lysate Lane 2: Ramos (Human Burkitt's lymphoma cell) cell lysate Lane 3: Raji (Human Burkitt's lymphoma cell) cell lysate Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601118, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 25 kDa Observed band size: 45-55 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD79a antibody (HA601118) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601118) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-CD79a antibody (HA601118) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601118) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Mouse anti-CD79a antibody (HA601118) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601118) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Tonsil Sample: Paraffin-embedded section Primary antibody dilution: 1/400 Antigen retrieval: ER2 Platform: Leica Biosystems BOND® RX |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.