CD32B Mouse Monoclonal Antibody [A9C11]
cat.: HA601121
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A9C11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human aa 1-250 / 310. |
| Positive control: | Daudi cell lysate, Raji cell lysate, HL-60 cell lysate, human placenta tissue lysate, human tonsil tissue, human liver tissue, human colon carcinoma tissue, human placenta tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P31994 Human |
| Alternative names: | CD32 CD32 antigen CD32B CDw32 Fc fragment of IgG, low affinity II, receptor for (CD32) Fc fragment of IgG, low affinity IIb, receptor Fc fragment of IgG, low affinity IIb, receptor (CD32) Fc fragment of IgG, low affinity IIb, receptor for (CD32) Fc fragment of IgG, low affinity IIb, receptor for Fc gamma receptor IIB Fc gamma RII Fc gamma RIIB Fc-gamma RII Fc-gamma RII-b Fc-gamma-RIIb FCG2 FCG2B_HUMAN Fcgr2 FCGR2B FCRII FcRII-b IGFR2 IgG Fc receptor II beta IgG Fc receptor II-b Low affinity immunoglobulin gamma Fc region receptor II Low affinity immunoglobulin gamma Fc region receptor II-b Ly-17 Lym 1 Lymphocyte antigen 17 |
Images
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Fig1:
Western blot analysis of CD32B on different lysates with Mouse anti-CD32B antibody (HA601121) at 1/1,000 dilution. Lane 1: Daudi cell lysate (10 µg/Lane) Lane 2: Raji cell lysate (10 µg/Lane) Lane 3: HL-60 cell lysate (10 µg/Lane) Lane 4: Human placenta tissue lysate (20 µg/Lane) Predicted band size: 34 kDa Observed band size: 39 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601121) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD32B antibody (HA601121) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601121) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD32B antibody (HA601121) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601121) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-CD32B antibody (HA601121) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601121) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-CD32B antibody (HA601121) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601121) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.