CD98 Mouse Monoclonal Antibody [A9D8]
cat.: HA601124
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A9D8 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human CD98 aa 450-630 (Extracellular). |
| Positive control: | A431 cell lysate, Hela cell lysate, 293T cell lysate, HepG2 cell lysate, human kidney tissue. |
| Subcellular location: | Apical cell membrane, Cell membrane, Cell junction, Lysosome membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P08195 Human |
| Alternative names: | 4F2 4F2 cell surface antigen heavy chain 4F2 cell-surface antigen heavy chain 4F2 heavy chain 4F2 heavy chain antigen 4F2_HUMAN 4F2hc 4T2HC Antigen defined by monoclonal 4F2 heavy chain Antigen identified by monoclonal antibodies 4F2 TRA1.10 TROP4 and T43 CD 98 CD98 CD98 antigen CD98 heavy chain CD98HC Heavy chain Lymphocyte activation antigen 4F2 large subunit MDU 1 MDU1 Monoclonal 44D7 NACAE Slc3a2 Solute carrier family 3 (activators of dibasic and neutral amino acid transport) member 2 Solute carrier family 3 member 2 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-CD98 antibody (HA601124) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601124) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Western blot analysis of CD98 on different lysates with Mouse anti-CD98 antibody (HA601124) at 1/1,000 dilution. Lane 1: A431(Human epidermoid carcinoma skin squamous cell) cell lysate Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 3: HEK-293T (Human embryonic kidney cell) cell lysate Lane 4: HepG2 (Human hepatocellular carcinoma cell) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute 22 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA601124, 1/1,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 68.0 kDa Observed band size: 80 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.