CD98 Mouse Monoclonal Antibody [A9D7]
cat.: HA601125
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A9D7 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 68 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human CD98 aa 450-630 (Extracellular). |
| Positive control: | A431 cell lysate, Hela cell lysate, HepG2 cell lysate, human skin tissue, human kidney tissue. |
| Subcellular location: | Apical cell membrane, Cell membrane, Cell junction, Lysosome membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P08195 Human |
| Alternative names: | 4F2 4F2 cell surface antigen heavy chain 4F2 cell-surface antigen heavy chain 4F2 heavy chain 4F2 heavy chain antigen 4F2_HUMAN 4F2hc 4T2HC Antigen defined by monoclonal 4F2 heavy chain Antigen identified by monoclonal antibodies 4F2 TRA1.10 TROP4 and T43 CD 98 CD98 CD98 antigen CD98 heavy chain CD98HC Heavy chain Lymphocyte activation antigen 4F2 large subunit MDU 1 MDU1 Monoclonal 44D7 NACAE Slc3a2 Solute carrier family 3 (activators of dibasic and neutral amino acid transport) member 2 Solute carrier family 3 member 2 |
Images
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Fig1:
Western blot analysis of CD98 on different lysates with Mouse anti-CD98 antibody (HA601125) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: Hela cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 68 kDa Observed band size: 75~100 kDa Exposure time: 1 minute 22 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601125) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-CD98 antibody (HA601125) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601125) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-CD98 antibody (HA601125) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601125) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.