PSMD6 Mouse Monoclonal Antibody [A9E4]
cat.: HA601127
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A9E4 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45.5 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human PSMD6 aa 1-150 / 389. |
| Positive control: | K562 cell lysate, MCF-7 cell lysate, HEK-293 cell lysate, Hela cell lysate, THP-1 cell lysate, human kidney tissue lysate, human testis tissue. |
| Subcellular location: | Cytosol, extracellular region, nucleoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q15008 Human |
| Alternative names: | 26S proteasome non-ATPase regulatory subunit 6 26S proteasome regulatory subunit RPN7 26S proteasome regulatory subunit S10 Breast cancer-associated protein SGA-113M KIAA0107 p42A P44S10 PFAAP4 Phosphonoformate immuno-associated protein 4 Proteasome (prosome, macropain) 26S subunit, non-ATPase, 6 Proteasome regulatory particle subunit p44S10 PSMD6 PSMD6_HUMAN Rpn7 S10 SGA-113M |
Images
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Fig1:
Western blot analysis of PSMD6 on different lysates with Mouse anti-PSMD6 antibody (HA601127) at 1/1,000 dilution. Lane 1: K562 cell lysate Lane 2: MCF-7 cell lysate Lane 3: HEK-293 cell lysate Lane 4: Hela cell lysate Lane 5: THP-1 cell lysate Lane 6: Human kidney tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 45.5 kDa Observed band size: 40 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601127) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-PSMD6 antibody (HA601127) at 5μg/mL dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601127) at 5μg/mL dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.