HA601128

Protein CASP Mouse Monoclonal Antibody [A9F6]

cat.: HA601128

Product Type:	Mouse monoclonal IgG1, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Cell
Clonality:	Monoclonal
Clone number:	A9F6

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 77 kDa
Isotype:	IgG1
Immunogen:	Recombinant protein within human Protein CASP aa 6-55.
Positive control:	SH-SY5Y cell lysate, 293T cell lysate, SK-Br-3 cell lysate, Hela cell lysate, MCF-7 cell lysate, K562 cell lysate, NIH/3T3 cell lysate, human kidney tissue lysate, human endometrium tissue, human kidney tissue, human small intestine tissue, rat brain tissue.
Subcellular location:	Golgi apparatus membrane.
Recommended Dilutions: WB IHC-P IF-Cell	1:5,000 1:200-1:1,000 1:200-1:1,000
Uniprot #:	SwissProt: Q13948 Human \| P70403 Mouse
Alternative names:	CASP_HUMAN CUX1 Protein CASP CCAAT displacement protein CDP CDP/Cut CDP1 Clox COY1 Cut homeobox Cut homolog Cut like 1 Cut like homeobox 1 CUTL1 CUX Cux/CDP CUX1 Golgi integral membrane protein 6 GOLIM6 Homeobox protein cux 1 Nbla10317 p100 p110 p200 p75 Putative protein product of Nbla10317

Images

Fig1: Western blot analysis of Protein CASP on different lysates with Mouse anti-Protein CASP antibody (HA601128) at 1/5,000 dilution.

Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: SK-Br-3 cell lysate (20 µg/Lane)
Lane 4: Hela cell lysate (20 µg/Lane)
Lane 5: Hela cell lysate (20 µg/Lane)
Lane 6: MCF-7 cell lysate (20 µg/Lane)
Lane 7: K562 cell lysate (20 µg/Lane)
Lane 8: 293T cell lysate (20 µg/Lane)
Lane 9: NIH/3T3 cell lysate (20 µg/Lane)
Lane 10: Human kidney tissue lysate (40 µg/Lane)

Predicted band size: 77 kDa
Observed band size: 77 kDa

Exposure time: 19 seconds;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601128) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

Fig2: Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Mouse anti-Protein CASP antibody (HA601128) at 1ug/mL dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601128) at 1ug/mL dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Protein CASP antibody (HA601128) at 1ug/mL dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601128) at 1ug/mL dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Mouse anti-Protein CASP antibody (HA601128) at 1ug/mL dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601128) at 1ug/mL dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Protein CASP antibody (HA601128) at 1ug/mL dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601128) at 1ug/mL dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig6: Immunocytochemistry analysis of Hela cells labeling Protein CASP with Mouse anti-Protein CASP antibody (HA601128) at 1ug/mL dilution.

Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Protein CASP antibody (HA601128) at 1ug/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Fig7: Immunocytochemistry analysis of SH-SY5Y cells labeling Protein CASP with Mouse anti-Protein CASP antibody (HA601128) at 1ug/mL dilution.

Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Protein CASP antibody (HA601128) at 1ug/mL dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.