Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
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Species reactivity: | Human |
Applications: | IHC-P |
Clonality: | Monoclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 10 kDa |
Isotype: | IgG1 |
Immunogen: | Synthetic peptide within Human Mammaglobin A aa 21-70 / 93. |
Positive control: | Human skin tissue, human breast tissue. |
Subcellular location: | Secreted. |
Recommended Dilutions:
IHC-P |
1:2,000 |
Uniprot #: | SwissProt: Q13296 Human |
Alternative names: | Mammaglobin 1 Mammaglobin-1 Mammaglobin-A MGB1 Scgb2a2 Secretoglobin family 2A member 2 SG2A2 SG2A2_HUMAN UGB2 |
Fig1:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-Mammaglobin A antibody (HA601136) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601136) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Mammaglobin A antibody (HA601136) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601136) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney (Negative) tissue with Mouse anti-Mammaglobin A antibody (HA601136) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601136) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |