HA601142

MUC1 Mouse Monoclonal Antibody [PSH0-49]

cat.: HA601142

Product Type:	Mouse monoclonal IgG2a, primary antibodies
Species reactivity:	Human
Applications:	IHC-P, FC, IF-Cell
Clonality:	Monoclonal
Clone number:	PSH0-49

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 122 kDa
Isotype:	IgG2a
Immunogen:	Synthetic peptide of core peptide domain of human MUC1.
Positive control:	Human breast carcinoma tissue, human kidney tissue, human lung tissue, human tonsil tissue, HeLa, SK-Br-3.
Subcellular location:	Apical cell membrane. Secreted. Nucleus, Cell membrane, Cytoplasm.
Recommended Dilutions: IHC-P FC IF-Cell	1:5,000-1:10,000 1:500-1:1,000 1:500
Uniprot #:	SwissProt: P15941 Human
Alternative names:	ADMCKD ADMCKD1 Breast carcinoma associated antigen DF3 Breast carcinoma-associated antigen DF3 CA 15-3 CA15 3 CA15 3 antigen CA15-3 CA15.3 Cancer antigen 15-3 Carcinoma associated mucin Carcinoma-associated mucin CD 227 CD227 DF3 antigen EMA Episialin Epithelial Membrane Antigen H23 antigen H23AG KL 6 KL-6 KL6 Krebs von den Lungen-6 MAM 6 MAM6 MCD MCKD MCKD1 Medullary cystic kidney disease 1 (autosomal dominant) Medullary cystic kidney disease, autosomal dominant MUC 1 MUC-1 MUC-1/SEC MUC-1/X MUC1 MUC1-alpha MUC1-beta MUC1-CT MUC1-NT MUC1/ZD MUC1_HUMAN Mucin 1 Mucin 1 cell surface associated Mucin 1 transmembrane Mucin 1, cell surface associated Mucin-1 subunit beta Peanut reactive urinary mucin Peanut-reactive urinary mucin PEM PEMT Polymorphic epithelial mucin PUM Tumor associated epithelial membrane antigen Tumor associated epithelial mucin Tumor associated mucin Tumor-ass......

Images

	Fig1: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-MUC1 antibody (HA601142) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601142) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-MUC1 antibody (HA601142) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601142) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue with Mouse anti-MUC1 antibody (HA601142) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601142) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-MUC1 antibody (HA601142) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601142) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunocytochemistry analysis of HeLa (positive) and HCT 116 (negative) labeling MUC1 with Mouse anti-MUC1 antibody (HA601142) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MUC1 antibody (HA601142) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
	Fig6: Flow cytometric analysis of SK-Br-3 cells labeling MUC1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601142, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Fig7: Flow cytometric analysis of HeLa cells labeling MUC1.

Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601142, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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