ACSL5 Recombinant Mouse Monoclonal Antibody [A9D4-R]
cat.: HA601149
Product Type: Recombinant Mouse monoclonal IgG1, primary antibodies
Species reactivity: Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: A9D4-R
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 76 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human aa 151-300 / 683.
Positive control: Mouse liver tissue lysate, rat liver tissue lysate, mouse liver tissue, rat liver tissue.
Subcellular location: Mitochondrion, Endoplasmic reticulum, Mitochondrion outer membrane, Endoplasmic reticulum membrane, Cell membrane.
Recommended Dilutions:
  WB
  IHC-P

1:1,000
1:1,000
Uniprot #: SwissProt: Q8JZR0 Mouse | O88813 Rat
Alternative names: ACS2 ACS5 Acyl CoA synthetase 5 Acyl CoA synthetase long chain family member 5 EC 6.2.1.3 FACL5 FACL5 for fatty acid coenzyme A ligase 5 Fatty acid CoA ligase, long chain 5 Fatty acid coenzyme A ligase 5 Fatty acid Coenzyme A ligase long chain 5 LACS 5 Long chain acyl CoA synthetase 5 Long chain fatty acid CoA ligase 5 Long chain fatty acid coenzyme A ligase 5
Images
HA601149_1.jpg Fig1: Western blot analysis of ACSL5 on different lysates with Mouse anti-ACSL5 antibody (HA601149) at 1/1,000 dilution.

Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 76 kDa
Observed band size: 70 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601149) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
HA601149_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-ACSL5 antibody (HA601149) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601149) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601149_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-ACSL5 antibody (HA601149) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601149) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.