Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
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Species reactivity: | Mouse, Rat |
Applications: | WB, IHC-P |
Clonality: | Monoclonal |
Clone number: | A9D4-R |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 76 kDa |
Isotype: | IgG1 |
Immunogen: | Recombinant protein within human aa 151-300 / 683. |
Positive control: | Mouse liver tissue lysate, rat liver tissue lysate, mouse liver tissue, rat liver tissue. |
Subcellular location: | Mitochondrion, Endoplasmic reticulum, Mitochondrion outer membrane, Endoplasmic reticulum membrane, Cell membrane. |
Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
Uniprot #: | SwissProt: Q8JZR0 Mouse | O88813 Rat |
Alternative names: | ACS2 ACS5 Acyl CoA synthetase 5 Acyl CoA synthetase long chain family member 5 EC 6.2.1.3 FACL5 FACL5 for fatty acid coenzyme A ligase 5 Fatty acid CoA ligase, long chain 5 Fatty acid coenzyme A ligase 5 Fatty acid Coenzyme A ligase long chain 5 LACS 5 Long chain acyl CoA synthetase 5 Long chain fatty acid CoA ligase 5 Long chain fatty acid coenzyme A ligase 5 |
Fig1:
Western blot analysis of ACSL5 on different lysates with Mouse anti-ACSL5 antibody (HA601149) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601149) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-ACSL5 antibody (HA601149) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601149) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-ACSL5 antibody (HA601149) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601149) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |