DDR2 Mouse Monoclonal Antibody [A9H5]
cat.: HA601150
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A9H5 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 97 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within human DDR2 aa 22-200 / 855. |
| Positive control: | HUVEC cell lysate, U-87 MG cell lysate, C2C12 cell lysate, HeLa cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q16832 Human | Q62371 Mouse |
| Alternative names: | CD167 antigen-like family member B CD167b CD167b antigen Cell migration inducing protein 20 DDR 2 DDR2 DDR2_HUMAN Discoidin domain containing receptor 2 Discoidin domain receptor 2 Discoidin domain receptor family member 2 discoidin domain receptor tyrosine kinase 2 Discoidin domain-containing receptor 2 discoidin domain-containing receptor tyrosine kinase 2 Hydroxyaryl protein kinase MIG20a Migration inducing gene 16 protein Neurotrophic tyrosine kinase Neurotrophic tyrosine kinase receptor related 3 NTRKR 3 NTRKR3 Receptor protein tyrosine kinase TKT Receptor protein-tyrosine kinase TKT Receptor related 3 receptor-related 3 TKT TYRO 10 TYRO10 Tyrosine kinase receptor related to neurotrophic TRK Tyrosine protein kinase TYRO 10 Tyrosine protein kinase TYRO10 Tyrosine-protein kinase TYRO10 Tyrosylprotein kinase |
Images
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Fig1:
Western blot analysis of DDR2 on different lysates with Mouse anti-DDR2 antibody (HA601150) at 1/1,000 dilution. Lane 1: HUVEC cell lysate Lane 2: MDA-MB-231 cell lysate (negative) Lane 3: U-87 MG cell lysate Lane 4: C2C12 cell lysate Lane 5: HeLa cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601150) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.