MVK Mouse Monoclonal Antibody [A9G12]
cat.: HA601151
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A9G12 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42.5 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human 197-396 / 396. |
| Positive control: | HepG2 cell lysate, Jurkat cell lysate, COS-1 cell lysate, SK-Br-3 cell lysate, HEK-293 cell lysate, K-562 cell lysate, MCF7 cell lysate, A431 cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, human kidney tissue, human liver tissue, human testis tissue, Jurkat. |
| Subcellular location: | Cytoplasm, Peroxisome. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:1,000 1:100 |
| Uniprot #: | SwissProt: Q03426 Human | Q9R008 Mouse | P17256 Rat |
| Alternative names: | FLJ96772 KIME_HUMAN LH receptor mRNA binding protein LRBP Mevalonate kinase 1 Mevalonate kinase Mevalonic aciduria MK mvk MVLK POROK3 |
Images
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Fig1:
Western blot analysis of MVK on different lysates with Mouse anti-MVK antibody (HA601151) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Jurkat cell lysate Lane 3: COS-1 cell lysate Lane 4: SK-Br-3 cell lysate Lane 5: HEK-293 cell lysate Lane 6: K-562 cell lysate Lane 7: MCF7 cell lysate Lane 8: A431 cell lysate Lane 9: PC-12 cell lysate Lane 10: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 42.5 kDa Observed band size: 42.5/130 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601151) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-MVK antibody (HA601151) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601151) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-MVK antibody (HA601151) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601151) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-MVK antibody (HA601151) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601151) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunocytochemistry analysis of Jurkat cells labeling MVK with Mouse anti-MVK antibody (HA601151) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MVK antibody (HA601151) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.