PAI1 Mouse Monoclonal Antibody [A9G8]
cat.: HA601152
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue, FC |
| Clonality: | Monoclonal |
| Clone number: | A9G8 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human PAI1 aa 31-80 / 402. |
| Positive control: | No heat NIH/3T3 cell lysate, no heat NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate, NIH/3T3 cell lysate, NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate, HUVEC cell lysate, mouse placenta tissue lysate, rat placenta tissue lysate, human breast carcinoma tissue, human placenta tissue, HepG2, NIH/3T3. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P IF-Tissue FC |
1:1,000 1:5,000 1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P05121 Human | P22777 Mouse | P20961 Rat |
| Alternative names: | Clade E Endothelial plasminogen activator inhibitor Nexin Nexin plasminogen activator inhibitor type 1 PAI 1 PAI PAI-1 PAI1_HUMAN PLANH1 Plasminogen activator inhibitor 1 Plasminogen activator inhibitor type 1 Serine (or cysteine) proteinase inhibitor Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 Serine proteinase inhibitor clade E member 1 serpin Serpin E1 Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 Serpin peptidase inhibitor clade E Serpine 1 SERPINE1 |
Images
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Fig1:
Western blot analysis of PAI1 on different lysates with Mouse anti-PAI1 antibody (HA601152) at 1/1,000 dilution. Lane 1: no heat NIH/3T3 cell lysate (20 µg/Lane) Lane 2: no heat NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 serum starved for 4 hours then treated with 10ng/mL TGF-β for 21 hours add 300ng/mL BFA for 18 hours cell lysate (20 µg/Lane) Lane 5: HUVEC cell lysate (20 µg/Lane) Lane 6: Mouse placenta tissue lysate (40 µg/Lane) Lane 7: Rat placenta tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 45/100 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601152) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (NBI02H) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-PAI1 antibody (HA601152) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601152) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-PAI1 antibody (HA601152) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601152) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunofluorescence analysis of paraffin-embedded human placenta tissue labeling PAI1 with Mouse anti-PAI1 antibody (HA601152) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601152, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig5:
Flow cytometric analysis of HepG2 cells labeling PAI1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601152, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig6:
Flow cytometric analysis of NIH/3T3 cells labeling PAI1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601152, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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