Human IL-3 Mouse Monoclonal Antibody [PSH0-96]
cat.: HA601153
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH0-96 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17.2 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant full length human IL-3 protein. |
| Positive control: | Recombinant human IL-3 protein |
| Subcellular location: | Secreted |
| Recommended Dilutions:
ELISA(Cap) |
1:500-1:1,000 |
| Uniprot #: | SwissProt: P08700 Human |
| Alternative names: | Colony stimulating factor multiple Hematopoietic growth factor IL 3 IL-3 IL3 IL3_HUMAN Interleukin 3 (colony stimulating factor, multiple) Interleukin 3 Interleukin-3 Mast cell growth factor MCGF MGC79398 MGC79399 Multi CSF Multilineage colony stimulating factor Multipotential colony stimulating factor Multipotential colony-stimulating factor OTTHUMP00000065963 P cell stimulating factor P-cell-stimulating factor |
Images
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Fig1:
Standard curve of human IL-3 matched pair antibodies: Sandwich ELISA analysis of human IL-3 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody [PSH0-96] diluted in carbonate/bicarbonate buffer, at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted IL-3 protein starting from 2000 pg/ml to 0 pg/ml and detect antibody [PSH0-98]-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.