SLC39A8 Mouse Monoclonal Antibody [A9G3]
cat.: HA601161
| Product Type: | Mouse monoclonal IgM, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A9G3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 49.6 kDa |
| Isotype: | IgM |
| Immunogen: | Recombinant protein within human SLC39A8 aa 23-132 (Extracellular). |
| Positive control: | Jurkat cell lysate, SiHa cell lysate, HeLa cell lysate, HUVEC cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9C0K1 Human | Q91W10 Mouse | Q5FVQ0 Rat |
| Alternative names: | 4933419D20Rik AA986696 BCG induced integral membrane protein 103 BCG induced integral membrane protein BIGMo-103 (Up-regulated by BCG-CWS) (Hypothetical protein) LIV-1 subfamily of ZIP zinc transporter 6 LZT Hs6 MGC108852 PP3105 SLC39A8 Solute carrier family 39 (zinc transporter), member 8 Solute carrier family 39 (zinc transporter), member 8 (SLC39A8) Solute carrier family 39 member 8 zinc transporter ZIP8 ZIP8 Zrt- and Irt-like protein 8 |
Images
|
Fig1:
Western blot analysis of SLC39A8 on different lysates with Mouse anti-SLC39A8 antibody (HA601161) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: SiHa cell lysate Lane 3: HeLa cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 50 kDa Observed band size: 65 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601161) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of SLC39A8 on different lysates with Mouse anti-SLC39A8 antibody (HA601161) at 1/1,000 dilution. Lane 1: HUVEC cell lysate (30 µg/Lane) Lane 2: NIH/3T3 cell lysate (30 µg/Lane) Lane 3: RAW264.7 cell lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (26 µg/Lane) Predicted band size: 50 kDa Observed band size: 65/130 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601161) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.