DAP Kinase 1 Recombinant Mouse Monoclonal Antibody [12B2-R]
cat.: HA601162
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 12B2-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 160 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human DAP Kinase 1 aa 451-700 / 1,430. |
| Positive control: | A549 cell lysate, TF-1 cell lysate, HepG2 cell lysate, human brain tissue lysate, human lung carcinoma tissue. |
| Subcellular location: | Cytoplasm, cytoskeleton. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:2,000 |
| Uniprot #: | SwissProt: P53355 Human |
| Alternative names: | DAK1 DAP K1 DAP kinase 1 DAPK 1 DAPK DAPK1 DAPK1_HUMAN Death Associated Protein Kinase 1 Death-associated protein kinase 1 DKFZp781I035 |
Images
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Fig1:
Western blot analysis of DAP Kinase 1 on different lysates with Mouse anti-DAP Kinase 1 antibody (HA601162) at 1/2,000 dilution. Lane 1: A549 cell lysate Lane 2: TF-1 cell lysate Lane 3: HepG2 cell lysate Lane 4: Human brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 160 kDa Observed band size: 160 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601162) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-DAP Kinase 1 antibody (HA601162) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601162) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.