CD204 Recombinant Mouse Monoclonal Antibody [A8G2-R]
cat.: HA601165
Product Type: Recombinant Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IHC-P, IF-Cell
Clonality: Monoclonal
Clone number: A8G2-R
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 0.85ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 50 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human CD204 aa 51-250 / 451.
Positive control: THP-1 treated with 50ng/mL PMA for 72 hours whole cell lysate, human liver tissue, human placenta tissue, human spleen tissue, rat liver tissue.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
1:1,000
1ug/mL-5ug/mL
1:100
Uniprot #: SwissProt: P21757 Human | P30204 Mouse
Entrez Gene: 498638 Rat
Alternative names: CD204 CD204 antigen CD24 Macrophage acetylated LDL receptor I and II Macrophage scavenger receptor 1 Macrophage scavenger receptor type III Macrophage scavenger receptor types I and II Msr 1 MSR1 MSRE_HUMAN phSR1 phSR2 SCARA 1 SCARA1 Scavenger receptor class A member 1 Scavenger receptor class A, member 1 Scavenger receptor type A Scvr SR A SRA
Images
HA601165_1.jpg Fig1: Western blot analysis of CD204 on different lysates with Mouse anti-CD204 antibody (HA601165) at 1/1,000 dilution.

Lane 1: THP-1 treated with 50ng/mL PMA for 72 hours whole cell lysate
Lane 2: THP-1 whole cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 50 kDa
Observed band size: 75 kDa

Exposure time: 50 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601165) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
HA601165_2.jpg Fig2: Immunocytochemistry analysis of THP-1 cells treated with or without 50ng/mL PMA for 72 hours labeling CD204 with Mouse anti-CD204 antibody (HA601165) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD204 antibody (HA601165) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
HA601165_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD204 antibody (HA601165) at 1μg/mL dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601165) at 1μg/mL dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601165_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-CD204 antibody (HA601165) at 1μg/mL dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601165) at 1μg/mL dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601165_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD204 antibody (HA601165) at 1μg/mL dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601165) at 1μg/mL dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA601165_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-CD204 antibody (HA601165) at 5μg/mL dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601165) at 5μg/mL dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.