ST2 Mouse Monoclonal Antibody [PSH01-68]
cat.: HA601170
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH01-68 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 63.4 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human ST2 aa 19-328 (Extracellular). |
| Positive control: | HeLa transfected with FLAG-tagged ST2 cell lysate, HeLa overexpress with ST2. |
| Subcellular location: | Cell membrane, Membrane, Secreted. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q01638 Human |
| Alternative names: | DER-4 DER4 FIT 1 Growth stimulation expressed homolog of mouse growth stimulation-expressed Il1rl1 IL33R ILRL1_HUMAN Interleukin 1 receptor like 1 interleukin 1 receptor related protein Interleukin-1 receptor-like 1 Protein ST2 ST2 ST2L ST2V T1 T1 protein |
Images
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Fig1:
Western blot analysis of ST2 on different lysates with Mouse anti-ST2 antibody (HA601170) at 1/1,000 dilution. Lane 1: HeLa transfected with FLAG-tagged empty control cell lysate Lane 2: HeLa transfected with FLAG-tagged ST2 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 63 kDa Observed band size: 80 kDa Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601170) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HeLa overexpress with or without ST2 cells labeling ST2 with Mouse anti-ST2 antibody (HA601170) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ST2 antibody (HA601170) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HeLa overexpress with or without ST2 cells labeling ST2. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601170, 1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 594 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1126) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.