SLC40A1 Mouse Monoclonal Antibody [A10A1]
cat.: HA601178
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A10A1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 0.87ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human SLC40A1 aa 257-306 / 571. |
| Positive control: | HEK-293 cell lysate, HepG2 cell lysate, A375 cell lysate, NCCIT cell lysate, Caco-2 cell lysate, SW480 cell lysate. |
| Subcellular location: | Cell membrane, Basolateral cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9NP59 Human |
| Alternative names: | Ferroportin 1 Ferroportin Ferroportin-1 FPN1 HFE4 IREG1 iron regulated gene 1 Iron regulated transporter 1 Iron-regulated transporter 1 MST079 MSTP079 MTP1 putative ferroportin 1 variant IIIA putative ferroportin 1 variant IIIB S40A1_HUMAN SLC11A3 SLC11A3, formerly SLC40A1 solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3 solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3, formerly solute carrier family 40 (iron-regulated transporter), member 1 Solute carrier family 40 member 1 |
Images
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Fig1:
Western blot analysis of SLC40A1 on different lysates with Mouse anti-SLC40A1 antibody (HA601178) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HepG2 cell lysate Lane 3: A375 cell lysate Lane 4: NCCIT cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601178) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of SLC40A1 on different lysates with Mouse anti-SLC40A1 antibody (HA601178) at 1/1,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: SW480 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601178) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.