SCD1 Recombinant Mouse Monoclonal Antibody [A9A11-R]
cat.: HA601180
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A9A11-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human SCD1 aa 310-509 / 509. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, HEK-293 cell lysate, SK-MEL-28 cell lysate, A375 cell lysate, A431 cell lysate, A549 cell lysate, mouse liver tissue lysate, rat liver tissue lysate. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O00767 Human | P13516 Mouse | P07308 Rat |
| Alternative names: | ACOD_HUMAN Acyl-CoA desaturase Delta(9)-desaturase Delta-9 desaturase Delta-9-Desaturase FADS5 Fatty acid desaturase PRO0998 SCD 1 SCD SCD1 Stearoyl-CoA desaturase |
Images
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Fig1:
Western blot analysis of SCD1 on different lysates with Mouse anti-SCD1 antibody (HA601180) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: HEK-293 cell lysate Lane 4: SK-MEL-28 cell lysate Lane 5: A375 cell lysate Lane 6: A431 cell lysate Lane 7: A549 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 42 kDa Observed band size: 37 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601180) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of SCD1 on different lysates with Mouse anti-SCD1 antibody (HA601180) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 42 kDa Observed band size: 37 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601180) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.