| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A10G5-R |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 586 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human MUC5AC aa 600-850. |
| Positive control: | Human stomach tissue, mouse stomach tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P |
1:1,000 |
| Uniprot #: | SwissProt: P98088 Human Entrez Gene: 17833 Mouse |
| Alternative names: | Apomucin Apomucin Major Airway Glycoprotein Gastric mucin LeB Lewis B blood group antigen Major Airway Glycoprotein MUC 5 MUC5 MUC5AC Mucin 5 subtype AC tracheobronchial Mucin 5 Subtypes A And C Mucin 5 subtypes A and C tracheobronchial/gastric Mucin 5, tracheobronchial/gastric Mucin 5AC Mucin 5AC oligomeric mucus/gel forming Mucin 5AC, oligomeric mucus/gel forming pseudogene Mucin TBM Tracheobronchial Mucin (TBM) Tracheobronchial mucin |
|
Fig1:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Mouse anti-MUC5AC antibody (HA601181) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601181) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Mouse anti-MUC5AC antibody (HA601181) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601181) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |