ddx24 Recombinant Mouse Monoclonal Antibody [A3B10-R]
cat.: HA601183
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Zebrafish |
| Applications: | IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | A3B10-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 94.5 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within zebrafish ddx24 aa 562-819 / 832. |
| Positive control: | Zebrafish tissue. |
| Subcellular location: | Nucleolus. |
| Recommended Dilutions:
IHC-P IF-Tissue |
1:1,000 1:50 |
| Uniprot #: | SwissProt: A0A0R4IA19 Zebrafish |
| Alternative names: | DEAD (Asp-Glu-Ala-Asp) box helicase 24 ddx24 |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded zebrafish (head) tissue labeling ddx24 with Mouse anti-ddx24 antibody (HA601183) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601183, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig2:
Immunofluorescence analysis of paraffin-embedded zebrafish (tail) tissue labeling ddx24 with Mouse anti-ddx24 antibody (HA601183) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601183, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded zebrafish tissue with Mouse anti-ddx24 antibody (HA601183) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601183) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.