HRP Conjugated Anti-DYKDDDDK Tag (FLAG) Recombinant Antibody [A2-A4-rFc] - Rabbit IgG (Chimeric)
cat.: HA601185
| Product Type: | Recombinant Chimeric Antibody IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB |
| Clone number: | A2-A4-rFc |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide immune sequence is N-DYKDDDDK-C. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Alternative names: | DDDDK epitope tag DDDK ddk DYKDDDDK DYKDDDDK epitope tag DYKDDDDK tag ECS epitope tag ECS tag Enterokinase Cleavage Site epitope tag Enterokinase Cleavage Site tag FLAG FLAG tag |
Images
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Fig1:
Western blot analysis of DYKDDDDK Tag ( FLAG ) on different lysates with Rabbit anti-DYKDDDDK Tag ( FLAG ) antibody (HA601185) at different dilutions. Lane 1: N-Flag at 1/1,000 dilution Lane 2: C-Flag at 1/5,000 dilution Lysates/proteins at 50 ng/Lane. Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601185) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.