LYRIC Recombinant Mouse Monoclonal Antibody [C6-C10-R]
cat.: HA601188
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | C6-C10-R |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 64 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human LYRIC aa 6-233 / 582. |
| Positive control: | HeLa cell lysate, PC-12 cell lysate, MCF7 cell lysate, Jurkat cell lysate, human breast carcinoma tissue, mouse brain tissue, Jurkat. |
| Subcellular location: | Nucleus membrane, nucleolus, endoplasmic reticulum membrane, tight junction, perinuclear region, cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:1,000 1:100 |
| Uniprot #: | SwissProt: Q86UE4 Human | Q80WJ7 Mouse | Q9Z1W6 Rat |
| Alternative names: | 3D3 3D3/LYRIC AEG 1 AEG-1 AEG1 Astrocyte elevated gene 1 Astrocyte elevated gene-1 protein LYRIC LYRIC/3D3 LYRIC_HUMAN Lysine rich CEACAM1 associated protein Lysine rich CEACAM1 co isolated protein Lysine-rich CEACAM1 co-isolated protein Metadherin Metastasis adhesion protein MTDH Protein LYRIC |
Images
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Fig1:
Western blot analysis of LYRIC on different lysates with Mouse anti-LYRIC antibody (HA601188) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: PC-12 cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Predicted band size: 64 kDa Observed band size: 75 kDa Exposure time: 53 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601188) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-LYRIC antibody (HA601188) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601188) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-LYRIC antibody (HA601188) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601188) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of Jurkat cells labeling LYRIC with Mouse anti-LYRIC antibody (HA601188) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-LYRIC antibody (HA601188) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.