Transmembrane protein 200A Recombinant Mouse Monoclonal Antibody [4-B3-R]
cat.: HA601191
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | 4-B3-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human Transmembrane protein 200A aa 59-359 / 491. |
| Positive control: | A549 cell lysate, Caco-2 cell lysate, PC-3M cell lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q86VY9 Human |
| Alternative names: | KIAA1913 T200A_HUMAN Tmem200a Transmembrane protein 200A TTMC Two transmembrane C |
Images
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Fig1:
Western blot analysis of Transmembrane protein 200A on different lysates with Mouse anti-Transmembrane protein 200A antibody (HA601191) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: Caco-2 cell lysate Lane 3: PC-3M cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 47 kDa Exposure time: 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601191) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.