LAMP2 Recombinant Mouse Monoclonal Antibody [D3-D8-D3-R]
cat.: HA601192
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | D3-D8-D3-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein corresponding to N terminal Human LAMP2. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, HUVEC cell lysate, JAR cell lysate, HEK-293 cell lysate, THP-1 cell lysate, human kidney tissue, human liver tissue. |
| Subcellular location: | Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:5,000 |
| Uniprot #: | SwissProt: P13473 Human |
| Alternative names: | CD107 antigen like family member B CD107 antigen-like family member B CD107b LAMP 2 LAMP 2C LAMP-2 LAMP2 LAMP2_HUMAN LAMPB LGP 96 LGP110 LGP96 Lysosomal associated membrane protein 2 Lysosome associated membrane glycoprotein 2 Lysosome associated membrane protein 2 Lysosome-associated membrane glycoprotein 2 Lysosome-associated membrane protein 2 MAC3 |
Images
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Fig1:
Western blot analysis of LAMP2 on different lysates with Mouse anti-LAMP2 antibody (HA601192) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HepG2 cell lysate Lane 4: HUVEC cell lysate Lane 5: JAR cell lysate Lane 6: HEK-293 cell lysate Lane 7: THP-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 110 kDa Exposure time: 3 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601192) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-LAMP2 antibody (HA601192) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601192) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-LAMP2 antibody (HA601192) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601192) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.