T7 tag Recombinant Mouse Monoclonal Antibody [4-C2-R]
cat.: HA601193
Product Type: Recombinant Mouse monoclonal IgG1, primary antibodies
Species reactivity: Species independent
Applications: WB
Clonality: Monoclonal
Clone number: 4-C2-R
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Isotype: IgG1
Immunogen: Synthetic peptide CMASMTGGQQMG.
Recommended Dilutions:
  WB

1:1,000
Alternative names: 10 Major capsid protein 10A Major capsid protein Minor capsid protein 10B T7 epitope tag
Images
HA601193_1.jpg Fig1: Western blot analysis of T7 tag on different T7 tagged recombinant proteins with Mouse anti-T7 tag antibody (HA601193) at 1/1,000 dilution.

Lysates/proteins at 50 ng/Lane.

Exposure time: 43 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601193) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.