alpha Actinin Recombinant Mouse Monoclonal Antibody [A1G2-R]
cat.: HA601194
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A1G2-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 103 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human ACTN1 aa 388-619 / 892. |
| Positive control: | HeLa cell lysate, A431 cell lysate, A549 cell lysate, HepG2 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, human breast tissue, human breast carcinoma tissue. |
| Subcellular location: | Cell membrane, cytoskeleton, Z line, cell junction, ruffle. |
| Recommended Dilutions:
WB IHC-P |
1:5,000 1:6,000 |
| Uniprot #: | SwissProt: P12814 Human | Q7TPR4 Mouse | Q9Z1P2 Rat |
| Alternative names: | actinin 1 smooth muscle Actinin alpha 1 actinin, alpha 1 ACTN 1 Actn1 ACTN1_HUMAN Alpha Actinin 1 Alpha actinin cytoskeletal isoform Alpha-actinin cytoskeletal isoform Alpha-actinin-1 BDPLT15 F actin cross linking protein F-actin cross-linking protein FLJ40884 FLJ54432 Non muscle alpha actinin 1 Non-muscle alpha-actinin-1 |
Images
|
Fig1:
Western blot analysis of alpha Actinin on different lysates with Mouse anti-alpha Actinin antibody (HA601194) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: HepG2 cell lysate Lane 5: MCF7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 103 kDa Observed band size: 103 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601194) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-alpha Actinin antibody (HA601194) at 1/6,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601194) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-alpha Actinin antibody (HA601194) at 1/6,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601194) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.