Human IL-21 Recombinant Mouse Monoclonal Antibody [PSH02-49]
cat.: HA601196
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH02-49 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18.6 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human IL-21 aa 25-162 (Q9HBE4). |
| Positive control: | Recombinant Human IL-21 Protein (HA211070). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [PSH02-48] to Human IL-21 (Capture) (HA601195). |
| Uniprot #: | SwissProt: Q9HBE4 Human |
| Alternative names: | CVID11 IL 21 IL-21 Il21 IL21_HUMAN Interleukin 21 Interleukin-21 interleukin-21 isoform Interleukin21 OTTHUMP00000164088 Za11 |
Images
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Fig1:
Sandwich ELISA analysis of Human IL-21 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA601195) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-21 (HA211070) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA601196, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.