Oct4 Recombinant Mouse Monoclonal Antibody [A3-C11-R]
cat.: HA601207
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A3-C11-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within full length human Oct4 protein. |
| Positive control: | NCCIT cell lysates, NCCIT. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q01860 Human |
| Alternative names: | Octamer binding transcription factor 4 MGC22487 Oct 3 Oct 4 Oct-3 Oct-4 OCT3 Oct4 Octamer binding protein 3 Octamer binding protein 4 Octamer binding transcription factor 3 Octamer-binding protein 3 Octamer-binding protein 4 Octamer-binding transcription factor 3 OTF 3 OTF 4 OTF-3 OTF3 OTF4 PO5F1_HUMAN POU class 5 homeobox 1 POU domain class 5 transcription factor 1 POU domain transcription factor OCT4 POU domain, class 5, transcription factor 1 POU-type homeodomain-containing DNA-binding protein POU5F1 |
Images
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Fig1:
Western blot analysis of Oct4 on NCCIT cell lysates with Mouse anti-Oct4 antibody (HA601207) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 39 kDa Observed band size: 45 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601207) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of NCCIT cells labeling Oct4 with Mouse anti-Oct4 antibody (HA601207) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Oct4 antibody (HA601207) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.