C10orf58 Recombinant Mouse Monoclonal Antibody [7-B7-R]
cat.: HA601210
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | 7-B7-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human FAM213A aa 1-229 / 229. |
| Positive control: | HUVEC cell lysate, SK-MEL-28 cell lysate, rat skin tissue lysate, SK-MEL-28. |
| Subcellular location: | Cytoplasm, Secreted . |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q9BRX8 Human | Q6AXX6 Rat |
| Alternative names: | Peroxiredoxin-like 2A PRXL2A Peroxiredoxin-like 2 activated in M-CSF stimulated monocytes 2 Publications Protein PAMM 2 Publications Redox-regulatory protein FAM213A C10orf58 FAM213A PAMM PRO2290 PSEC0139 UNQ611/PRO1198 |
Images
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Fig1:
Western blot analysis of C10orf58 on different lysates with Mouse anti-C10orf58 antibody (HA601210) at 1/2,000 dilution. Lane 1: HUVEC cell lysate (20 µg/Lane) Lane 2: SK-MEL-28 cell lysate (20 µg/Lane) Lane 3: Rat skin tissue lysate (40 µg/Lane) Predicted band size: 25 kDa Observed band size: 24/25 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601210) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of SK-MEL-28 cells labeling C10orf58 with Mouse anti-C10orf58 antibody (HA601210) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-C10orf58 antibody (HA601210) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of SK-MEL-28 cells labeling C10orf58. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601210, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.