CD35 Recombinant Mouse Monoclonal Antibody [A4F3-R]
cat.: HA601217
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A4F3-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 224 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within C-terminal human CD35. |
| Positive control: | TF-1 cell lysate, human kidney tissue lysate, human tonsil tissue, human Hodgkin lymphoma tissue, human small intestine tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:2,000 1:200 1:1,000 |
| Uniprot #: | SwissProt: P17927 Human |
| Alternative names: | C3 binding protein C3b/C4b receptor C3BR C4BR CD 35 CD35 CD35 antigen complement component (3b/4b) receptor 1 (Knops blood group) complement component (3b/4b) receptor 1 including Knops blood group system Complement component receptor 1 Complement receptor 1 Complement receptor type 1 CR 1 CR1 CR1_HUMAN KN Knops blood group antigen |
Images
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Fig1:
Western blot analysis of CD35 on different lysates with Mouse anti-CD35 antibody (HA601217) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Human kidney tissue lysate Lane 3: K-562 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 224 kDa Observed band size: 280 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601217) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling CD35 with Mouse anti-CD35 antibody (HA601217) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601217, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human Hodgkin lymphoma tissue with Mouse anti-CD35 antibody (HA601217) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601217) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Mouse anti-CD35 antibody (HA601217) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601217) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD35 antibody (HA601217) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601217) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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