ZAP70 Recombinant Mouse Monoclonal Antibody [A1B5-R]
cat.: HA601218
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A1B5-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human ZAP70 aa 250-480. |
| Positive control: | Jurkat cell lysate, mouse spleen tissue lysate, mouse thymus tissue lysate, rat spleen tissue lysate, rat thymus tissue lysate, human tonsil tissue. |
| Subcellular location: | Cell membrane, cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:2,000 |
| Uniprot #: | SwissProt: P43403 Human | P43404 Mouse Entrez Gene: 301348 Rat |
| Alternative names: | 70 kDa zeta associated protein 70 kDa zeta-associated protein EC 2.7.10.2 FLJ17670 FLJ17679 Selective T cell defect SRK STD Syk related tyrosine kinase Syk-related tyrosine kinase Truncated ZAP kinase Tyrosine protein kinase ZAP70 Tyrosine-protein kinase ZAP-70 TZK ZAP 70 ZAP70 ZAP70_HUMAN Zeta chain associated protein kinase 70kD Zeta chain associated protein kinase 70kDa Zeta chain associated protein kinase 70kDa isoform 1 Zeta chain associated protein kinase 70kDa isoform 2 Zeta chain of T cell receptor associated protein kinase 70 Zeta chain TCR associated protein kinase 70kD Zeta chain TCR associated protein kinase 70kDa |
Images
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Fig1:
Western blot analysis of ZAP70 on different lysates with Mouse anti-ZAP70 antibody (HA601218) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Ramos cell lysate (negative) Lane 3: Mouse spleen tissue lysate Lane 4: Mouse thymus tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat thymus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601218) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-ZAP70 antibody (HA601218) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601218) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.