PDCD6 Recombinant Mouse Monoclonal Antibody [A6A7-R]
cat.: HA601219
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | A6A7-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human PDCD6 aa 1-191 / 191. |
| Subcellular location: | Endosome, Nucleus, Endoplasmic reticulum membrane, Cytoplasm, COPII-coated vesicle membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: O75340 Human |
| Alternative names: | AIP1 ALG 2 ALG-2-interacting protein 1 ALG2 ALIX Apoptosis linked gene 2 Apoptosis linked gene 2 protein Apoptosis-linked gene 2 protein FLJ42309 FLJ46208 Hp95 KIAA1375 MA 3 MA3 MGC111017 MGC119050 MGC9123 PDCD 6 Pdcd6 PDCD6_HUMAN PEF 1B PEF1B Probable calcium binding protein ALG 2 Probable calcium binding protein ALG2 Probable calcium-binding protein ALG-2 Programmed cell death 6 Programmed cell death 6-interacting protein Programmed cell death protein 6 PS 2 PS2 |
Images
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Fig1:
Western blot analysis of PDCD6 on different lysates with Mouse anti-PDCD6 antibody (HA601219) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HepG2 cell lysate Lane 3: 293T cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601219) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling PDCD6 with Mouse anti-PDCD6 antibody (HA601219) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-PDCD6 antibody (HA601219) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HeLa cells labeling PDCD6. Cells were fixed and permeabilized. Then stained with the primary antibody (HA601219, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.