HA601222

BrdU Recombinant Antibody [PSH0-18]

cat.: HA601222

Product Type:	Recombinant Chimeric Antibody IgG1, primary antibodies
Species reactivity:	Species independent
Applications:	IHC-P, IF-Cell, IF-Tissue
Clone number:	PSH0-18

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Isotype:	IgG1
Immunogen:	BrdU-OVA
Positive control:	BrdU treated mouse embryo brain tissue, BrdU treated mouse embryo cartilage tissue, BrdU treated mouse embryo liver tissue, BrdU treated NIH/3T3.
Subcellular location:	Nucleus.
Recommended Dilutions: IHC-P IF-Cell IF-Tissue	1:10,000 1:50 1:1,000
Alternative names:	Bromodeoxyuridine BUdr

Images

	Fig1: Immunohistochemical analysis of paraffin-embedded mouse embryo brain tissue (Brdu treated / Untreated / Edu treated) with Mouse anti-BrdU antibody (HA601222) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601222) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig2: Immunohistochemical analysis of paraffin-embedded mouse embryo cartilage tissue (Brdu treated / Untreated / Edu treated) with Mouse anti-BrdU antibody (HA601222) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601222) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig3: Immunohistochemical analysis of paraffin-embedded mouse embryo liver tissue (Brdu treated / Untreated / Edu treated) with Mouse anti-BrdU antibody (HA601222) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601222) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells (Brdu treated / Untreated / Edu treated) with Mouse anti-BrdU antibody (HA601222) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA601222) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunocytochemistry analysis of NIH/3T3 cells (Untreated / Brdu treated / Edu treated) labeling BrdU with Mouse anti-BrdU antibody (HA601222) at 1/50 dilution. Cells were fixed in 70% ethyl alcohol for 5 minutes at room temperature, then subjected to acid hydrolysis using 2M HCL in TBST for 30 minutes at room temperature. permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-BrdU antibody (HA601222) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
	Fig6: Immunofluorescence analysis of paraffin-embedded mouse embryo brain tissue (Untreated / Brdu treated / Edu treated) labeling BrdU with Mouse anti-BrdU antibody (HA601222) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601222, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Fig7: Immunofluorescence analysis of paraffin-embedded mouse embryo cartilage tissue (Untreated / Brdu treated / Edu treated) labeling BrdU with Mouse anti-BrdU antibody (HA601222) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601222, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

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