NuMA Mouse Monoclonal Antibody [PSH03-08]
cat.: HA601224
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH03-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 238 kDa kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human NuMA aa 1-200 / 2,115 (HA210958). |
| Positive control: | Recombinant Human NuMA protein (HA210958). |
| Subcellular location: | Nucleus, Cytoskeleton, Plasma membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [PSH03-07] to Human NuMA (Capture) (HA601223) and Recombinant Human NuMA protein (HA210958) as the standard. The reference range value is 51-12500 pg/ml. |
| Uniprot #: | SwissProt: Q14980 Human |
| Alternative names: | Centrophilin stabilizes mitotic spindle in mitotic cells NMP 22 Nuclear matrix protein 22 Nuclear mitotic apparatus protein 1 Nuclear mitotic apparatus protein NUMA 1 NUMA NuMA protein NUMA1 NUMA1_HUMAN SP H antigen SP-H antigen Structural nuclear protein |
Images
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Fig1:
Sandwich ELISA analysis of human NuMA matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA601223) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant human NuMA protein (HA210958) starting from 30000 pg/ml to 0 pg/ml and detect antibody (HA601224, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native NuMA in Hela extract samples based on a 1000 µg/ml extract load. Interpolated concentration of native NuMA was measured in duplicate at different sample concentrations and interpolated from the NuMA standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean NuMA concentration was determined to be 5,378 pg/mL in Hela cell extract. |
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Fig3:
Interpolated concentrations of spiked NuMA in cell culture media samples. The concentrations of NuMA were measured in duplicates, interpolated from the NuMA standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.