NuMA Mouse Monoclonal Antibody [PSH03-08]
cat.: HA601224
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH03-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 238 kDa kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human NuMA aa 1-200 / 2,115. |
| Subcellular location: | Nucleus, Cytoskeleton, Plasma membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [PSH03-07] to Human NuMA (Capture) (HA601223). The reference range value is 51-12500 pg/ml. |
| Uniprot #: | SwissProt: Q14980 Human |
| Alternative names: | Centrophilin stabilizes mitotic spindle in mitotic cells NMP 22 Nuclear matrix protein 22 Nuclear mitotic apparatus protein 1 Nuclear mitotic apparatus protein NUMA 1 NUMA NuMA protein NUMA1 NUMA1_HUMAN SP H antigen SP-H antigen Structural nuclear protein |
Images
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Fig1: The concentrations of NUMA1 were interpolated from the NUMA1 standard curve and corrected for sample dilution. The mean NUMA1 concentration was determined to be 1,238 pg/ml in HeLa cell extract, 832 pg/ml in MCF7 cell extract and 1,360 pg/ml in K562 cell extract. |
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Fig2:
Sandwich ELISA analysis of human NUMA1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA601223) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human NUMA1 protein starting from 12500 pg/ml to 0 pg/ml and detect antibody (HA601224)-Biotin (0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.