Alpha 1 Acid Glycoprotein Recombinant Mouse Monoclonal Antibody [A4-G2-A3-R]
cat.: HA601226
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | A4-G2-A3-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human ORM1 aa 1-201 / 201. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, HCT 116 cell lysate, 293T cell lysate, human liver tissue lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P02763 Human |
| Alternative names: | A1AG1_HUMAN AGP 1 AGP A AGP AGP1 Alpha 1 acid glycoprotein Alpha-1-acid glycoprotein 1 alpha-1-AGP Epididymis secretory sperm binding protein Li 153w glycoprotein, alpha-1-acid, of serum HEL S 153w OMD 1 ORM ORM1 Orosomucoid 1 Orosomucoid-1 |
Images
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Fig1:
Western blot analysis of Alpha 1 Acid Glycoprotein on different lysates with Mouse anti-Alpha 1 Acid Glycoprotein antibody (HA601226) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: HCT 116 cell lysate (20 µg/Lane) Lane 4: 293T cell lysate (20 µg/Lane) Lane 5: Human liver tissue lysate (40 µg/Lane) Predicted band size: 24 kDa Observed band size: 37/45 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601226) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.