Cdk4 Recombinant Mouse Monoclonal Antibody [5-C3-G3-R]
cat.: HA601227
| Product Type: | Recombinant Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | 5-C3-G3-R |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human Cdk4 aa 80-303/303. |
| Positive control: | A549 cell lysate, 293T cell lysate, HeLa cell lysate, MCF7 cell lysate, K-562 cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus, Nucleus membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P11802 Human | P30285 Mouse |
| Alternative names: | Cdk 4 cdk4 CDK4 protein CDK4_HUMAN Cell division kinase 4 Cell division protein kinase 4 CMM 3 CMM3 Crk3 Cyclin dependent kinase 4 Cyclin-dependent kinase 4 Melanoma cutaneous malignant 3 MGC14458 p34 cdk4 PSK J3 PSK-J3 |
Images
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Fig1:
Western blot analysis of Cdk4 on different lysates with Mouse anti-Cdk4 antibody (HA601227) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: HeLa cell lysate Lane 4: MCF7 cell lysate Lane 5: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601227) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.